|本期目录/Table of Contents|

脂肪因子ZAG在耐力运动改善大鼠胰岛素抵抗中的作用

《山东体育学院学报》[ISSN:1006-2076/CN:37-1013/G8]

期数:
2019年04期
页码:
0
栏目:
出版日期:
2019-08-25

文章信息/Info

Title:
-
作者:
韩雨梅1罗昕1冀里栋2田俊生3秦雪梅3李建英1
1.山西大学体育学院,山西 太原030006;2. 山西医科大学汾阳学院,山西 汾阳032200;3.山西大学中医药现代研究中心,山西 太原030006
Author(s):
-
关键词:
ZAGPGC-1α肝脏胰岛素抵抗耐力运动
Keywords:
-
分类号:
G804.2
DOI:
-
文献标识码:
A
摘要:
目的:研究肝脏ZAG在耐力运动改善高脂饮食诱导的大鼠胰岛素抵抗中的作用机制。方法:雄性 Wistar大鼠32只随机分成安静对照组(N)、运动对照组(Ne)、IR安静组(IR)和IR运动组(IRe)。高脂饲料喂养建模(IR)5周,高胰岛素-正葡萄糖钳夹试验验证模型成功。运动干预6周,转轮运动,20 m/min,45 min/天,5天/周。6周运动末禁食12 h后再次钳夹试验评价胰岛素抵抗状态,次日脱臼处死,全自动生化分析仪检测大鼠CHOL、HDL-CHOL、LDL-CHOL、TG水平,ELISA检测血清FINS,Western-blot检测肝脏ZAG和PGC-1a蛋白表达。结果:1)5周高脂饲料喂养成功诱导大鼠IR模型;2)IR组FINS、CHOL和TG显著高于N组,GIR显著低于N组,呈现IR的代谢特点;Ne组比N组大鼠体重极显著下降,FINS显著提高。IRe组比IR组大鼠体重、FINS均显著下降,GIR极显著性提高。3)IR组大鼠肝脏ZAG和PGC-1α蛋白表达均显著低于N组,Ne组和IRe组大鼠肝脏ZAG和PGC-1α蛋白表达均显著高于各自安静对照组。4)大鼠肝脏ZAG蛋白表达量与HDL-C、GIR、PGC-1α蛋白表达量显著正相关,与CHOL、TG、FINS显著负相关。结论:1)耐力运动可以提高IR大鼠肝脏ZAG和PGC-1α蛋白表达,改善血脂代谢,改善IR状态。2)耐力运动过程中大鼠肝脏ZAG可能通过促进线粒体生物合相关因子PGC-1α表达而发挥刺激脂肪分解的作用。
Abstract:
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备注/Memo

备注/Memo:
中国博士后科学基金资助课题(2014M560197;2015T80234)。
更新日期/Last Update: 1900-01-01